TELL-Seq™ Whole Genome Sequencing Library Prep Kit – Long-Read Power with Short-Read Platforms

The TELL-Seq™ Whole Genome Sequencing (WGS) Library Prep Kit is a revolutionary technology that delivers long read sequencing capability using standard short read sequencers. It combines the advantages of linked-read barcoding with a simple, single-tube workflow, making it ideal for whole genome assembly, structural variant detection, and haplotype phasing.

Powered by Transposase Enzyme Linked Long-read Sequencing (TELL-Seq) technology, this kit enables:

•     De novo genome assembly
•     Structural variant analysis
•     Phasing and haplotype resolution
•     High-quality results from as little as 0.1 ng of input DNA

Compatible with Illumina® sequencers, TELL-Seq bridges the gap between long-read insight and short-read accessibility—without needing new instrumentation.

Description: 

The next generation of sequencing has arrived with Universal Sequencing’s TELL-SEQ™ Whole Genome Sequencing (WGS) Library Prep Kit. This new technology changes the idea of sequencing with a solution that is easy, scalable, and universal.

• Long read NGS library preparation method
• Ease of use: hands-on time ( < 1 hour)
• Single tube process: sequencing ready library in 3 hours
• Low DNA input: 0.1ng to 5ng depending on genome size
• Highly scalable for small and large genomes, 20 Kb to 5 Gb
• Easily Automated

Simple and scalable genome analysis

UST’s Transposase Enzyme-Linked Long-read Sequencing (UST TELL-Seq™) is a simple and scalable NGS library technology that generates barcode linked-reads for genome-scale sequencing applications. The whole procedure can be carried out in a PCR tube without the need for expensive instrumentation. The TELL-Seq™ Whole Genome Sequencing (WGS) Library Prep Kit will generate an Illumina sequencing library in just 3 hours. The protocol can be easily adjusted based on the genome size to be analyzed. TELL-Seq will be the new standard library method for WGS.

For genome sizes from 1MB to 5GB

Standard Bundle

The standard bundle has enough reagent to prepare 4 sample libraries for genomes 1 Gb in size or larger or 12 sample libraries for small genomes up to 200 Mb in size for both diploid and haploid genomes.

The standard bundle requires the following items:

• UST TELL-Seq™ WGS Library Reagent Box 1 & Box 2,
• UST TELL-Seq™ Library Multiplex Primer Kit,
• UST TELL-Seq™ Illumina® Sequencing Primer Kit

High-Throughput Bundle (HT24)

The high-throughput bundle has enough reagent to prepare 24 sample libraries of 1 Gb or larger in size or 72 sample libraries up to 200 Mb in size for both diploid and haploid genomes. 

The high-throughput bundle requires the following items:

• UST TELL-Seq™ WGS Library Reagent HT24 Box 1 & Box 2,
• UST TELL-Seq™ Library Multiplex Primer Kit (1-8)
• UST TELL-Seq™ Library Multiplex Primer Kit (9-16)
• UST TELL-Seq™ Library Multiplex Primer Kit (17-24)
• UST TELL-Seq™ Illumina® Sequencing Primer Kit (HT24)

• Enables long read sequencing using short read sequencers
• Based on TELL-Seq™ barcoding technology
• Requires only a single-tube, 3-hour workflow
• Works with low-input DNA (0.1–5 ng)
• Delivers ultra-long molecule information (50–200 Kb)
• Ideal for WGS, de novo assembly, SV detection & phasing
• Compatible with standard Illumina® platforms
• No microfluidics or specialized equipment required

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