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For all gene expression studies using qPCR, it is necessary to compensate for differences between samples due to material losses, differences in RT yields and PCR inhibition. Normalization should include a reference gene, but can also be complemented by identical sample-input amounts. The reference gene should have constant expression in all the samples compared. There is no universal control gene, expressed at a constant level under all conditions and in all tissues, hence the choice of reference gene should be validated for each new study.The best way to choose the proper reference gene(s) is by running a panel of potential genes on a number of representative test samples. The most appropriate gene(s) for normalization are chosen in each case.Content
The Reference Gene Panels are available for Human, Mouse and Rat. Genes with varying cellular functions and expression levels have been selected to reduce the risk of using co-regulated genes. The primers have been designed to be exon-spanning where possible, and to give low levels of primer dimers
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