Reference Gene Panel – Identify the Best qPCR Normalization Gene

The TATAA Reference Gene Panel – Standard Assays is a powerful tool for researchers aiming to perform accurate and reproducible gene expression analysis using real-time PCR. This panel contains a curated set of commonly used reference (housekeeping) genes, allowing you to evaluate and select the most stable gene for normalization in your specific biological sample and experimental condition.

Rather than assuming one reference gene fits all, this panel helps you test multiple genes in parallel, enabling evidence-based, data-driven qPCR normalization using the best internal control.

Description

For all gene expression studies using qPCR, it is necessary to compensate for differences between samples due to material losses, differences in RT yields and PCR inhibition. Normalization should include a reference gene, but can also be complemented by identical sample-input amounts. The reference gene should have constant expression in all the samples compared. There is no universal control gene, expressed at a constant level under all conditions and in all tissues, hence the choice of reference gene should be validated for each new study.
The best way to choose the proper reference gene(s) is by running a panel of potential genes on a number of representative test samples. The most appropriate gene(s) for normalization are chosen in each case.

Content

• Assays for 12 of the most common reference genes for gene expression studies
• Short assays contains primer sets for 9 potential reference genes in humans.
• Enough for 100 reactions per gene (25 µl qPCR reaction)
• Positive Control DNA

The Reference Gene Panels are available for Human, Mouse and Rat. Genes with varying cellular functions and expression levels have been selected to reduce the risk of using co-regulated genes. The primers have been designed to be exon-spanning where possible, and to give low levels of primer dimers.

• Panel includes 12 commonly used human reference genes
• Optimized for SYBR® Green qPCR detection
• Designed for reference gene screening across diverse samples
• Ensures accurate gene expression normalization
  

User Manual

Single assays from the panel

Other quality controls from TATAA Biocenter