AlphaThera oYo-Link® Antibody Labeling – Just 30 Seconds Hands-On

Label your antibodies in under 30 seconds of hands-on time with AlphaThera’s oYo-Link® technology, a revolutionary, site-specific labeling method that simplifies and accelerates the conjugation process.

Designed for researchers who value time, reproducibility, and flexibility, oYo-Link allows for UV-activated conjugation of a wide range of payloads (fluorophores, enzymes, oligos, etc.) to native antibodies—without needing purification or formulation changes.

Two Steps, 30 Seconds Hands-on Time, 100% Sample Recovery

The oYo-Link® Conjugation Technology can be used to site-specifically and covalently label the Fc region of almost any “off-the-shelf” antibody with click chemistry tags (e.g. Azide, DBCO), epitope tags, oligonucleotides, biotin and more.

oYo-Link reagents consist of low molecular weight (8 to 10 kDa), high-affinity antibody-binding domains that possess a photo-crosslinker within their Fc-binding site. Upon illumination with non-damaging Black-light, oYo-Link forms a covalent bond with the antibody.

SureLight® Antibody Labeling with AlphaThera
Label your antibodies quickly and effortlessly using AlphaThera’s oYo-Link® SureLight® reagents, including SureLight® APC and SureLight® PE. This UV-activated, site-specific labeling system allows for direct conjugation of fluorescent dyes to native antibodies—with only 30 seconds of hands-on time and no need for purification or buffer exchange. Ideal for flow cytometry and other fluorescence-based assays, SureLight® labeling ensures high signal intensity, low background, and consistent performance every time.

Antibody-Oligo Conjugates with AlphaThera
Create high-quality antibody-oligo conjugates in minutes using AlphaThera’s oYo-Link® technology. This site-specific, UV-activated labeling system enables direct attachment of single- or double-stranded DNA oligonucleotides, up to 80 bases long, to native antibodies. With no need for antibody modification, purification, or buffer exchange, it’s the fastest and most reliable method for generating conjugates for CITE-seq, immuno-PCR, spatial profiling, and other high-resolution applications.

Epitope Tags:

• His12
• DYKDDDDK (FLAG)
• V5
• S
• VSVg
• NWS
• S1
• AU1
• AU5
• HSV1

Chemical Conjugation Tags:

• Azide
• DBCO 
  
• Tetrazine
• Thiol 

Oligonucleotide Conjugation

• Custom oligos

Fluorescent and Enzymatic Labels:

• SureLight® APC
• SureLight® PE
• HRP (Horseradish Peroxidase)

Surface Immobilization Tags:

• His12 Tag
  
• Single-Biotin 

Antibody-Drug Conjugation (ADC) Payloads:

• DM1
• DM4
• VcMMAE
• VcMMAF

Other: 

• Hands-on time: 30 seconds or less
• AlphaThera’s proprietary oYo-Link® platform
• UV-activated conjugation without altering antibody formulation
• Works with commercially available antibodies
• Label with enzymes, fluorophores, DNA, oligos, etc.
• No purification or antibody modification required
• High reproducibility with site-specific labeling

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