ArcticZymes T4 DNA Ligase – Recombinant, High-Purity DNA/RNA Ligase

ArcticZymes T4 DNA Ligase is a high-quality, recombinant ATP- and Mg²⁺-dependent ligase designed for efficient ligation of DNA and RNA substrates. It catalyzes the formation of phosphodiester bonds between 3’-hydroxyl and 5’-phosphate termini in blunt-end and cohesive-end duplex DNA, duplex RNA, and DNA/RNA hybrids.

Produced in E. coli and stringently tested, this commercial-grade ligase is free from detectable DNase, RNase, and residual host genomic DNA contamination. It can be fully inactivated by incubation at 70°C for 10 minutes, ideal for workflows requiring thermal shutdown.

Description: 

T4 DNA Ligase is an ATP and Mg2+ dependent dsDNA ligase which catalyses the formation of a phosphodiester bond between 3’-hydroxyl and 5’-phosphate termini in duplex DNA, duplex RNA and some DNA/RNA hybrids. T4 DNA Ligase is active on both blunt-end and cohesive-end substrates.

This is a high-quality (commercial grade) version of the T4 DNA Ligase. T4 DNA Ligase is recombinantly produced in E. coli. ArcticZymes’ T4 DNA Ligase is extensively tested for contaminating DNase and RNase activities as well as residual host-cell gDNA.

• Recombinant T4 DNA Ligase from ArcticZymes
• ATP and Mg²⁺-dependent phosphodiester bond formation
• Active on blunt and cohesive ends in DNA, RNA, and hybrids
• Fully inactivated by heat treatment at 70°C for 10 minutes
• Free from contaminating DNase, RNase, and host gDNA
• High-purity enzyme for cloning, ligation, and NGS library prep

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