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Vendor: Norgen Biotek
The Norgen FFPE RNA Purification Kit enables the efficient extraction of high-quality RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples, commonly used in clinical and pathology research.
This kit uses a proprietary buffer system to reverse formalin cross-linking, followed by a column-based purification process that delivers RNA suitable for RT-qPCR, RNA-Seq, microarray, and archival sample analysis.
Optimized to preserve RNA integrity and remove inhibitors, the kit supports reliable gene expression profiling from challenging clinical specimens.
Description:
For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samplesExtract total RNA (including microRNA) from FFPE samplesNo phenol extraction stepIncludes DNase for optional on-column DNA removalIsolated RNA is of the highest quality and integrityIsolate a diversity of RNA speciesFFPE RNA Purification Kit
Norgen’s FFPE RNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time.
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