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Vendor: Cellecta
The Cellecta CRISPR Cas9 Test Assay Kit enables precise detection of genome editing outcomes, including insertions and deletions (indels), caused by CRISPR-Cas9 and other gene-editing systems.
This ready-to-use CRISPR Cas9 kit provides a fast and reliable qPCR-based method for quantifying editing efficiency in a wide range of sample types—including transfected cell lines, primary cells, and animal models.
Designed and validated by Cellecta, a leader in functional genomics, the kit includes target-specific primers, controls, and reagents for high sensitivity and reproducibility in genome modification analysis.
Description:
Accurately Assess Cas9 Activity in Cells
The CRISPR-Test assay kits contain a mix of lentiviral particles with both green fluorescent and red fluorescent protein (GFP and RFP). The GFP fluorescent marker responds to the specific Cas9 activity the Kit measures whether nuclease (CRISPRu or CRISPR KO), gene repression (CRISPRi), or gene activation (CRISPRa). The RFP fluorescence provides a normalization control.
How Does it Work?
1.Transduce your test population of putative Cas9, dCas9-activator, or dCas9-repressor cells.
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