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Vendor: Norgen Biotek
The Norgen microRNA Purification Kit is designed for the efficient and selective isolation of microRNAs (miRNAs) and other small RNAs from a wide range of sample types including tissues, cultured cells, biofluids, and FFPE.
The kit’s proprietary chemistry ensures excellent recovery of small RNA species while minimizing contamination from large RNAs and DNA.
Ideal for downstream applications such as miRNA-Seq, RT-qPCR, and biomarker discovery, this kit provides high-quality miRNA suitable for expression analysis and translational research. Includes 25 preps.
Description:
Rapid purification of microRNA without phenol; isolation and extraction of microRNAExtract high quality & purity miRNA in 30 minutesTwo column kit- eliminate large RNA on first column and capture microRNA on a second columnElute microRNA in 25 µL ready for miRNA profilingProcess a wide range of samples- cell, tissue, bacteria, bodily fluids, etc
microRNA Purification KitThis kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, bacterial cells, plants and blood.
Two columns are provided with this kit. The first column captures large RNA, while the small RNA are captured on a second column and are eluted concentrated in 25 µL of nuclease-free water.
The small RNA can be used in various downstream applications relating to miRNA profiling, gene regulation and functional analysis. The eluted RNA is ready for RT-qPCR, microarrays, and NGS applications.
BackgroundThese small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation.
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