Norgen Cell Culture Media Exosome Purification and RNA Isolation MIDI Kit – 25 Preps

The Norgen Cell Culture Media Exosome Purification and RNA Isolation MIDI Kit is designed for the medium-throughput isolation of exosomes and their RNA cargo from conditioned cell culture media.

Optimized for studies in extracellular vesicle communication, exosomal RNA profiling, and biomarker discovery, this kit delivers consistent results from larger sample volumes.

Extracted RNA is compatible with downstream applications such as RT-qPCR, RNA-Seq, and small RNA analysis. Each kit contains 25 preps and is ideal for labs working with larger-scale EV studies.

Description: 

Purification and enrichment of intact cell culture media exosomes for functional studies
Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA?
Bind and elute all RNA irrespective of size or GC content, without bias
Versatile cell culture media input volume (10 mL - 20 mL)
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents nor overnight incubation required
Pure exosomes are purified and are free-from any other RNA-binding proteins
Cell Culture Media Exosome Purification and RNA Isolation Midi Kit

Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Midi Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes ranging from 10 mL to 20 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

• Developed by Norgen Biotek
• Captures exosomes and exosomal RNA from cell culture media
• Suited for larger volumes and medium-throughput workflows
• Ideal for RNA-Seq, RT-qPCR, and small RNA analysis
• Preserves exosome integrity and RNA quality
• Perfect for EV communication and secretome analysis
• Includes 25 preps per kit

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