TATAA GrandScript cDNA FreePrime Kit – Flexible cDNA Synthesis with Custom Priming

The TATAA GrandScript cDNA FreePrime Kit is a high-quality, flexible cDNA synthesis kit optimized for custom reverse transcription workflows. Unlike pre-primed mixes, this formulation is provided without oligo(dT) or random hexamer primers, allowing users to add their own gene-specific or custom primers.

Ideal for applications requiring precision and control over the reverse transcription process, the FreePrime Kit delivers robust, reproducible results across a broad range of RNA inputs.

Robust and sensitive cDNA synthesis with user-defined RT priming

The TATAA GrandScript cDNA Freeprime Kit provides all the necessary components for cDNA synthesis conveniently separated into different tubes, giving the user freedom to setup the RT priming strategy suitable for your application.

The TATAA GrandScript cDNA Freeprime Kit provides a robust, consistent and unbiased first strand synthesis over a broad range of RNA template concentrations. The cDNA product is directly compatible with quantitative real-time PCR methods. Random sequence and oligo(dT) primers are provided in separate tubes. User can also choose to use gene specific primers or Two-tailed primers, or a combination of the different priming strategies.

The TATAA GrandScript RT enzyme is a mixture of engineered M-MLV Reverse transcriptase and ribonuclease inhibitor.

Content

• TATAA GrandScript FreePrime reaction mix (5x concentrated solution of optimised buffer, magnesium, dNTPs and stabilizers)
• TATAA GrandScript RT enzyme
• Oligo dT (10x conc. Solution of oligo(dT)20 with enhancer
• Random primer (10x conc. Solution of random primer with enhancer)
• GSP Enhancer
• Nuclease-free water

Description

• Supplied without primers – use your own custom or gene-specific primers
• Optimized for high-yield and full-length cDNA synthesis
• Works with low- and high-input RNA, including degraded samples
• Ideal for RT-qPCR, targeted expression analysis, and custom assays

User Manual

Other cDNA synthesis kits from TATAA Biocenter