Norgen Cell Culture Media Exosome Purification and RNA Isolation Mini Kit – 50 Preps

The Norgen Cell Culture Media Exosome Purification and RNA Isolation Mini Kit enables the isolation of intact extracellular vesicles (EVs) and high-quality RNA from conditioned media.

This kit is ideal for researchers studying secreted RNA, exosomal communication, and cell signaling, especially in cancer, immunology, or regenerative medicine.

The protocol ensures efficient exosome capture and RNA purification for downstream applications such as RT-qPCR, RNA-Seq, and small RNA profiling. Includes 50 preps.

Description: 

Purification and enrichment of intact cell culture media exosomes for functional studies
Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA?
Bind and elute all RNA irrespective of size or GC content, without bias
Versatile cell culture media input volume (5 mL - 10 mL)
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents nor overnight incubation required
Pure exosomes are purified and are free-from any other RNA-binding proteins
Cell Culture Media Exosome Purification and RNA Isolation Mini Kit

Norgen's Cell Culture Media Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes ranging from 5 mL to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

• Manufactured by Norgen Biotek
• Isolates exosomes and exosomal RNA from cell culture media
• Ideal for studying secreted RNA, EVs, and cellular communication
• Compatible with RNA-Seq, RT-qPCR, and small RNA profiling
• Preserves RNA integrity from extracellular vesicles
• Suitable for biomarker discovery and translational research
• Includes 50 preps per kit

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