Hoechst 33342 & 333258 DNA Stains – Biotium

Biotium’s Hoechst 33342 and Hoechst 333258 are classic, cell-permeant blue fluorescent DNA stains that bind preferentially to A-T–rich regions in the minor groove of double-stranded DNA. They are widely used for nuclear staining, cell cycle studies, and apoptosis assays in both live and fixed cells.

These high-purity Biotium formulations offer bright nuclear staining, low background, and excellent compatibility with DAPI filter sets, making them ideal for:

•     Fluorescence microscopy
•     Flow cytometry
•     High-content screening
•     Cell viability and proliferation studies

More: 

•     Hoechst 33342 and 333258 available from Biotium
•     Excitation/emission: ~350/461 nm
•     Cell-permeable and suitable for live or fixed cell imaging
•     Preferential binding to A-T–rich DNA regions
•     Compatible with DAPI filter cubes
•     Ideal for nuclear visualization and cell cycle analysis
•     Trusted for fluorescence microscopy and cytometry workflows

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