Human Single Assay – qPCR Assay for Reference Genes

The TATAA Human Single Assay is designed for researchers who have already identified the most suitable reference gene for their study and now require a reliable and efficient qPCR assay to quantify it. This assay is tailored for SYBR® Green detection or probe, and provides high sensitivity and specificity, making it ideal for consistent and accurate gene expression normalization.

Description

Whether you're conducting routine experiments or expanding a study, the Human Single Assay delivers reproducible results across a wide range of human sample types.

For accurate gene expression measurements it is necessary to normalize results of the expression of target genes to a reference that is not affected by the parameters studied in the specific study. There exists to our knowledge no reference gene that is unaffected in all conditions. Therefore it is necessary to find the optimal reference gene for your conditions and to validate that this gene is indeed nonregulated. After a screening for suitable reference genes by using the TATAA Reference Gene Panel, single assays from the panels can be purchased separately.

• Designed for SYBR® Green real-time PCR or probe base quantification
• Select from commonly used human reference genes
• High efficiency and specificity
• Suitable for longitudinal studies and repeated use

User Manual

TATAA's reference gene selection kit