TATAA SYBR® GrandMaster® Mix – High-Specificity SYBR Green Master Mix for qPCR

TATAA SYBR® GrandMaster® Mix is a high-quality, ready-to-use 2X master mix designed for real-time PCR using SYBR Green chemistry. It contains a hot-start DNA polymerase, dNTPs, buffer, and SYBR Green I dye, all optimized to deliver excellent sensitivity, reproducibility, and specificity.

Developed by TATAA Biocenter, this mix ensures minimal primer-dimer formation and background fluorescence, making it ideal for both routine and demanding qPCR applications.

Run your qPCR with maximum efficiency, sensitivity and specificity

The TATAA SYBR® GrandMaster® Mix is a 2x concentrated ready to use fastmix which provides all the necessary components for qPCR, except template and primers. TATAA SYBR® GrandMaster® Mix delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR® Green qPCR.

The TATAA SYBR® GrandMaster® Mix is user friendly, does not form bubbles and has an outstanding stability: 6 months in 4°C and 3 months in room temperature.

Suggested applications

• Gene expression
• Single-cell analysis
• Small input amounts
• Other challenging applications

Features

• High efficiency for sensitive qPCR analysis
• Optimised for lower risk of primer-dimer formation
• Compatible with most qPCR instruments (ROX dye available)
• Compatible with the PCR Decontamination Kit (ArcticZymes) for 16S targets
• Robust fluorescent signal using fast, or conventional, cycling protocols with SYBR® Green qPCR 2x concentrated ready to use fastmix

Content

TATAA SYBR® GrandMaster Mix (2x): 2x reaction buffer containing optimized concentrations of: MgCl2 dNTPs Taq DNA polymerase SYBR® Green I Dye Stabilizers

 The number of reactions is based on 20μl-qPCR reactions.

Overview

•     High-specificity hot-start polymerase minimizes non-specific amplification
•     Optimized for low background and minimal primer-dimer signal
•     Suitable for a broad range of targets and template types
•     Compatible with all major real-time PCR instruments
•     Ideal for gene expression, validation assays, and quantification

Applications

•     Gene expression analysis
•     cDNA quantification
•     Primer validation
•     SYBR Green-based screening assays
•     Educational and clinical research workflows

User Manual

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