MALBAC® Single Cell Whole Genome Amplification Kit – Yikon Genomics

The MALBAC® Single Cell WGA Kit by Yikon Genomics enables robust and uniform whole genome amplification (WGA) from as little as a single cell. Built on the Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) technology, this kit minimizes amplification bias and preserves genomic integrity—ideal for downstream analysis like NGS, CNV detection, and single-cell genomics.

Trusted in both research and clinical settings, MALBAC is especially effective for:

•     Single-cell sequencing
•     Preimplantation genetic testing (PGT)
•     Copy number variation (CNV) analysis
•     Cancer genomics and rare cell analysis

Whole Genome Amplification for Single Cell(s), Picogram to Sub-nanogram Quantities of gDNA

Features:
Quasi-linear amplification with the least amplification bias
High sequencing coverage and uniformity
Low allele dropout rate
Single tube sample preparation in 4 hours
Suitable for single-cell CNV/SNV analysis, PGS/PGD, and CTC studies

Overview
Based on the patented MALBAC™ technology from Harvard University, MALBAC™ Single Cell WGA kit provides rapid, reliable and reproducible whole genome amplification of single cell(s) or picogram to sub-nanogram quantities of gDNA to produce 2-4 micrograms of amplified products in about 4 hours.

The kit is able to achieve consistent amplification efficiency in both AT- and GC-rich regions for most cell types, such as blastomere, polar body, sperm and various cell lines. More than 90% of genomic loci can be amplified and analyzed using different platforms, such as real-time PCR and next-generation sequencing.

• Based on proprietary MALBAC® technology
• Developed and manufactured by Yikon Genomics
• Enables uniform whole genome amplification from 1–5 cells
• Compatible with NGS, aCGH, qPCR, and CNV assays
• Minimizes sequence-dependent amplification bias
• Delivers high genome recovery and low allelic dropout
• Proven performance in PGT, cancer research, and single-cell analysis

User manual

Sequencing services