ArcticZymes dsDNase – Double-Strand Specific DNase for Clean RNA Prep

dsDNase from ArcticZymes is a double-strand specific DNase that selectively degrades dsDNA, leaving single-stranded DNA (ssDNA) and RNA intact.

It’s the perfect tool for removing contaminating genomic DNA from RNA preparations prior to RT-qPCR, RNA-Seq, or other transcriptomics workflows where high RNA integrity and DNA-free samples are critical. This high-purity enzyme integrates seamlessly into standard cleanup protocols and is easy to inactivate.

Description: 

dsDNase is inactivated at 65°C.

This is an all-purpose double-strand specific DNase by ArcticZymes, which is used in our PCR decontamination kit.

Heat inactivation

dsDNase can be heat inactivated by heat treatment at 20 min at 60?C, or 10 min at 65?C, in the presence of 1 mM DTT.

Specifications

Unit definition

One Unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCl2 (Kunitz, 1950).

Specific activity

475 000 Kunitz Units/mg.
pH optimum
pH 7.5 (in Tris-HCl).
Storage buffer
20 mM Tris-HCl pH 7.5, 2 mM MgCl2, 10 mM NaCl, 0.01% (v/v) Triton X-100, 50% (v/v) glycerol.
Reaction buffer
20 mM Tris-HCl pH 8.0, 5 mM MgCl2. Dependent on magnesium ions for activity. Dependent on 1 mM DTT for inactivation.

Storage

Minimum shelf life is 2 years at -20°C. Storage at 4°C is possible for at least 6 months. The enzyme also tolerates multiple freeze-thaw cycles.

• Recombinant dsDNase from ArcticZymes
• Selectively degrades double-stranded DNA (dsDNA)
• Preserves RNA and single-stranded DNA
• Ideal for transcriptomic workflows, including RT-qPCR and RNA-Seq
• Removes genomic DNA contamination for better sensitivity
• Easily inactivated and compatible with standard lab protocols

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For dsDNase Triton FREE 2-100k and dsDNase Triton FREE 50-50k: Contact Us