CelluLyser™ Micro Lysis Buffer – Rapid Cell Lysis for qPCR and RT-qPCR

CelluLyser™ Micro Lysis Buffer is a rapid and gentle lysis solution optimized for releasing nucleic acids from small numbers of cultured cells or cell pellets. Designed for workflows requiring direct input into qPCR or RT-qPCR, it enables fast and reliable RNA or DNA analysis without the need for column-based purification.

The buffer is optimized to maintain nucleic acid integrity while minimizing PCR inhibitors, making it ideal for sensitive applications such as gene expression studies, biomarker discovery, and single-cell analysis.

Fast and easy cell lysis of 1-500 cells

The CelluLyser™ Micro lysis buffer has been de novo designed to provide improved mRNA retrieval even for very low expressed genes, enhanced stabilization of mRNA, minimal degree of dilution and high compatibility with downstream reagents. The buffer can be combined with our cDNA synthesis kit. By generating cDNA directly from cell lysate without any washing steps higher yields are achieved, and to avoid losses of material the entire procedure can be performed in a single tube (or 96/384-well plates) without any sample transfer. The CelluLyser™ Micro lysis reagent has been developed for fast and simple lysis for single cell applications including very small samples (FACS sorted single cells, cell cultures, dissociated tissue).

Description

The CelluLyser™ Micro's properties make direct lysis and analysis of small cell numbers more accurate, flexible and feasible.

The CelluLyser™ Micro buffer has been developed for fast and simple cDNA preparation for single cell applications including very small samples (FACS sorted single cells, cell cultures, dissociated tissue).

Features

• Lysis buffer optimized for direct cell lysis from a single cell up to 500 cells in only 5 µl volume
• Optimized to preserve and stabilize released RNA
• Compatible with downstream reagents for reverse transcription
• Compatible with TATAA Universal RNA and DNA spikes for quality control of the workflow

Cell types

The CelluLyser™ Micro buffer has been developed to be a mild lysis solution. It has been verified on many types of in vitro and in vivo viable cells including astrocytes, microglia, fibroblasts, lymphocytes (B-cells, T-cells), leukemia cell lines (K562, HEK293), monocytes, breast cancer cells, sarcoma cell lines and embryonic stem cells.

Contents

• CelluLyserTM Micro buffer: 200 rxn à 5 µl, 1.0 ml (1X)

Overview

• Rapid lysis of cultured cells in micro-volume formats
• Compatible with both qPCR and RT-qPCR workflows
• No RNA or DNA purification required
• Preserves nucleic acid quality for accurate quantification
• Ideal for low-input or high-throughput screening applications

User Manual

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