TATAA Universal DNA Spike II – Synthetic Spike-In Control for Extraction & Amplification Monitoring

The TATAA Universal DNA Spike II is a high-purity synthetic DNA control designed to evaluate extraction efficiency, PCR performance, and inter-assay variability in nucleic acid workflows. It can be spiked into samples prior to extraction or into the PCR reaction, helping researchers identify sample loss, contamination, or inhibition.

With a unique, non-human DNA sequence, it ensures no interference with the sample's genetic material, making it ideal for complex biological matrices, including plasma, urine, or FFPE tissue.

Control for Inhibition of the qPCR - the risk of compromised RT-qPCR results

The Universal DNA Spike from TATAA Biocenter is easy to use and an very effective tool for quality control of the qPCR experimental workflow. DNA Spike is used in the qPCR reaction to detect any inhibition. In addition TATAA Universal RNA Spike I and II can be used to detect any inhibition during the cDNA synthesis reaction.

TATAA Universal DNA Spike II which has a DNA sequence length of 2000 base pairs. The Spike II assay which is used for detecting the Universal DNA Spike II template, is very robust and optimized for high sensitivity for inhibition. The assay amplifies a 286-base region towards the 3’ end of the synthetic template. The measured Cq-values and the shape of the amplification curves reflect the inhibition. The Cq-values of the Spike II assay also reflect losses during extraction, handling, transport, and storage of samples, including freeze-thaw events during RT-qPCR.

Key Benefits

•     Detects variability in sample prep and amplification
•     Internal QC to assess PCR inhibition and extraction consistency
•     Optimized fragment length and sequence for qPCR/dPCR compatibility
•     Enhances data reproducibility in regulated and research environments
•     Developed by TATAA Biocenter with validated quality and stability

Applications

•     Internal control for extraction workflows
•     Monitoring assay reproducibility (inter-assay variability)
•     Suitable for qPCR, dPCR, and other DNA quantification assays
•     Compatible with cfDNA and FFPE samples

User Manual

All DNA spikes from TATAA Biocenter