TATAA Universal DNA Spike I - Internal Spike-In Control for DNA Assay Performance Monitoring

The TATAA Universal DNA Spike I is a synthetic DNA control fragment (1000 bp) designed for use as an internal spike-in control during nucleic acid extraction and amplification workflows. With a unique sequence and consistent performance, it enables scientists to:

•     Detect sample-to-sample variation
•     Monitor inter-assay consistency
•     Identify inhibition or loss during extraction
•     Ensure accurate quantification in low-input or complex samples

Tailored for qPCR, digital PCR, or NGS workflows, this spike-in enhances reliability across a wide variety of applications, including liquid biopsy, FFPE tissue analysis, and pharmacogenomics.

Control for Inhibition of the qPCR - the risk of compromised RT-qPCR results

The Universal DNA Spike from TATAA Biocenter is an easy to use and very effective tool for quality control of the qPCR experimental workflow. DNA Spike is used in the qPCR reaction to detect any inhibition. In addition TATAA Universal RNA Spike I and II can be used to detect any inhibition during the cDNA synthesis reaction.

TATAA Universal DNA Spike for quality Control

The Universal DNA Spike has a synthetic sequence that is not present in any known living organism. This DNA sequence is 1000 base pairs in length. The Spike assay, which is very robust and is optimized for high sensitivity for inhibition, amplifies a 300-base region towards the 3’ end of the synthetic transcript. The measured Cq and the shape of the amplification curve reflect inhibition. If the Cq value is greater in an experimental sample than in the control then the analytical process of that experimental sample is inhibited. The magnitude of the difference between these Cq values reflects the degree of inhibition.

Applications

•     Internal spike-in control for extraction and amplification
•     Inter-assay variability assessment
•     Liquid biopsy and cfDNA workflows
•     Method development and QC for regulated assays

User Manual

All DNA spikes from TATAA Biocenter