TATAA Universal DNA Spike 500 bp – Internal Control for DNA Quantification and Normalization

TATAA Universal DNA Spike 500 bp is a high-quality, synthetic double-stranded DNA spike-in control designed to improve accuracy, reproducibility, and normalization in molecular biology workflows.

The 500 bp spike-in is ideal for use in qPCR, PCR, and DNA extraction validation, helping researchers control for variability in sample input, processing, or assay performance.

Unlike endogenous controls, this spike-in has no homology with human, mouse, or common model organisms, making it a reliable external reference across all sample types.

Control for qPCR-inhibition in samples with free circulating DNA or in degraded material 

The Universal DNA Spike from TATAA Biocenter is easy to use and an very effective tool for quality control of the qPCR experimental workflow. DNA Spike is used in the qPCR reaction to detect any inhibition.

In addition TATAA Universal RNA Spike I and II can be used to detect any inhibition during the cDNA synthesis reaction. In cases where predominantly short fragments of DNA may be present such as in free circulating DNA or in heavily degraded material a shorter DNA spike template , like Spike 166 bp or Spike 500 bp, may more correctly resemble DNA present in samples.

The Universal Spikes from TATAA Biocenter are offered as RNA or DNA templates in different synthetic sequences that are not present in any known living organism and can be used as very effective tools for quality control throughout the entire RT-qPCR experimental workflow.

TATAA Universal DNA Spike 500 bp has a DNA sequence length of 500 base pairs. T The Spike 500 bp assay which is used for detecting the Universal DNA Spike 500 bp template, is very robust and optimized for high sensitivity for inhibition. The assay amplifies a 81-base region of the synthetic template. The measured Cq-values and the shape of the amplification curves reflect the inhibition. The Cq-values of the Spike 500 bp assay also reflect losses during extraction, handling, transport, and storage of samples, including freeze-thaw events during RT-qPCR.

• DNA spike-in for normalization and sample comparison
• Designed to control for variability in DNA extraction and amplification
• Ideal for qPCR and PCR assay optimization
• No cross-reactivity with genomic DNA from common model species
• Developed by TATAA Biocenter to ensure research-grade performance

User Manual

 All DNA spikes from TATAA Biocenter