TATAA Probe GrandMaster® Mix – Universal qPCR Mastermix for Probe assays

TATAA Probe GrandMaster® Mix is a 2X universal qPCR mastermix developed for probe-based real-time PCR applications, including TaqMan® assays and hydrolysis probes.

Engineered for high sensitivity, fast cycling, and robust reproducibility, this mastermix simplifies qPCR workflows while maintaining top performance across instruments and targets.

Its proprietary formulation contains all essential components except primers, probes, and template, making it ideal for both routine diagnostics and advanced research.

Run your qPCR with ultimate sensitivity and efficiency

Description
The TATAA Probe GrandMaster® Mix is a 2x concentrated ready to use fastmix which provides all the necessary components for qPCR except template, primers and probe. The blue color of the mix helps to visualize pipetting in clear or white plates.

The TATAA Probe GrandMaster® Mix provides a robust and versatile solution for ultimate sensitivity and efficiency. It is compatible with a variety of fluorogenic probe chemistries including TaqMan® hydrolysis probes as well as an ultra pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA.

Features

• 2x concentrated ready to use fastmix
• Ultimate sensitivity and efficiency
• Compatible with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes
• Compatible with the PCR Decontamination Kit (ArcticZymes) for 16S targets
• A blue color to help visualize pipetting in clear or white plates
• Compatible with most qPCR instruments (ROX and Low ROX dye available)

Content
TATAA Probe GrandMaster® Mix (2x): 2x reaction buffer containing optimized concentrations of:

• MgCl2
• dNTPs
• hot-start DNA polymerase
• blue qPCR dye
• Stabilizers

The number of reactions is based on 20μl-qPCR reactions.

• Ready-to-use 2X mix for fast and reproducible qPCR results
• Compatible with all hydrolysis probe formats (e.g., TaqMan®)
• High sensitivity and wide dynamic range
• Suitable for gene expression, genotyping, and viral detection
• Validated across leading qPCR platforms (e.g., Bio-Rad, Roche, ABI)

User Manual

More quality reagents from TATAA Biocenter