TATAA PreAmp GrandMaster® Mix – High-Efficiency Preamplification for qPCR Workflows

TATAA PreAmp GrandMaster® Mix is a high-performance, ready-to-use mastermix designed specifically for uniform preamplification of multiple targets prior to qPCR analysis. It ensures excellent sensitivity and linearity, making it ideal for workflows with low RNA or cDNA input, such as single-cell analysis, FFPE samples, and rare transcript detection.

The optimized formulation minimizes bias and preserves the relative abundance of targets during preamplification, enabling more reliable downstream quantification.

Run your pre-amplification with ultimate efficiency and sensitivity 

TATAA PreAmp GrandMaster® Mix is a 2x concentrated ready to use pre-amplification mix for small samples, when looking at low expressed genes, single cells or when highly concentrated RNA is needed. The mix provides all the necessary components for pre-amplification of cDNA prior to qPCR, except template and primers. With the specialized TATAA PreAmp GrandMaster® Mix you will have the possibility to pre-amplify targets without the need of limiting or variable primer concentrations.

Features

PCR based pre-amplification Ultimate sensitivity and efficiency

Content

TATAA PreAmp GrandMaster® Mix (2x): reaction buffer containing optimized concentrations of: MgCl2 dNTPs Taq DNA polymerase Stabilizers

• The number of reactions is based on 50μl-qPCR reactions.
•     Uniform amplification of multiple qPCR targets
•     Enables accurate quantification from low-input samples
•     Optimized for high specificity and low background
•     Preserves target ratios across preamplification
•     Validated for compatibility with TATAA GrandMaster® qPCR mixes and commercial assays

Applications

•     Single-cell expression profiling
•     FFPE sample preamplification
•     Low-abundance target detection
•     Multiplex assay preparation
•     Preamplification before qPCR or digital PCR

User Manual

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