TATAA Multiplex GrandMaster® Mix – High-Efficiency Mastermix for Multiplex qPCR

TATAA Multiplex GrandMaster® Mix is a universal 2X mastermix specifically designed for high-performance multiplex qPCR applications. With a proprietary hot-start DNA polymerase and optimized buffer system, this mix enables accurate and efficient amplification of multiple targets in the same reaction—without cross-interference or signal loss.

Whether you’re developing gene expression panels, viral or microbial detection assays, or diagnostic multiplex workflows, this mix delivers outstanding reproducibility, sensitivity, and minimal background.

Go beyond multiplex limitations and achieve unbiased amplification
Description

TATAA Multiplex GrandMaster® Mix is a 2x concentrated ready to use multiplex mix. It provides all the necessary components for multiplex qPCR except template, primers and probes.

Conventional PCR master mixes provides limitations when it comes to multiplexing. With the specialized TATAA Multiplex GrandMaster® Mix you will have the possibility to multiplex up to five targets without the need of limiting or variable primer concentrations, with the same dynamic range and sensitivity as with singleplex assays.

Features

Real-time multiplex detection of up to 5 targets
Linearity over 6 orders of magnitude of input cDNA and 4 orders of magnitude of input genomic DNA
Detect up to 4 targets when one differs in expression up to 106-fold relative to the others

Content

• TATAA Multiplex GrandMaster Mix (2x): 2x reaction buffer containing optimized concentrations of:
• MgCl2
• dNTPs
• Taq DNA polymerase
• Stabilizer

Overview

• The number of reactions is based on 20µl-qPCR reactions.
• Optimized for multiplex qPCR with up to 5 targets
• Excellent sensitivity and specificity across a wide dynamic range
• Compatible with all major qPCR platforms
• Robust performance with complex samples like FFPE or crude lysates
• Fast cycling compatibility for reduced run times

Applications

•     Gene expression profiling
•     Viral/bacterial detection
•     Diagnostic panel development
•     Clinical biomarker studies
•     Pathogen screening and surveillance

User Manual

More quality reagents from TATAA Biocenter