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Vendor: TATAA Biocenter AB
Preamplification primer mix of GrandPerformance assays The Preamplification primer mix contains primers for all the GrandPerformance assay targets that will amplify them all in proportion for downstream singleplex qPCR analysis. The same Preamplification primer mix is used for all GrandPerformance assays combinations.
Background When sample amount is limited in gene expression profiling, or when certain qPCR technologies like the BioMark™ (Fluidigm) is to be used, a preamplification of the samples is advised in order to obtain high enough contentration of each target in a reaction to be able to produce reproducible data. The GrandPerformance Cancer PreAmp Primer Mix is a premixed preamplification solution of primers to be used together with the GrandPerformance Cancer Panel Probe qPCR assays for gene expression analysis. Other products recommended to be used together with the PreAmp Primer mix:
Volume: 250 µl Conc.: 10X