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Vendor: TATAA Biocenter AB
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Rapid and sensitive lysis and reverse transcription of small cell samples in a single tube
The optimized CelluLyser™ buffer and powerful reverse transcription reagents maximize the yield of cDNA, making CelluLyser™ ideal for studies of small samples intended for real-time PCR. With the possibility to generate cDNA directly from cell lysate without isolating RNA, considerable amount of time is saved compared to standard RNA purification.
The CelluLyser™ Lysis and cDNA Synthesis kit enables you to analyze small samples from 10 000 cells down to a single cell with minimal losses and hands-on time. The CelluLyser™ kit can be used with collected cells (FACS sorted, patch clamp collected cells etc.), as well as cultured cells (both adherent and in solution), and has successfully been tested on a number of different mammalian cell lines.
To avoid losses of material the entire procedure can be performed in a single tube without any sample transfer. Also, 100% of the total RNA content may be used from lysis to cDNA synthesis. The CelluLyser™ procedure is rapid and straightforward. Cells are lysed in only 10 minutes in the optimized CelluLyser™ buffer, and thereafter sensitive cDNA synthesis is performed directly on the cell lysate.
The reagents provided are sufficient either for 250 lysis reactions and 100 cDNA synthesis
reactions of 1 - 2 000 cells, or for 100 lysis reactions and 40 cDNA synthesis reactions
of 2 000 - 10 000 cells.
CelluLyserTM buffer: 1.25 ml (1X)
cDNA synthesis reagents (Roche Transcriptor First Strand cDNA Synthesis kit):
Transcriptor Reverse Transcriptase 50 µl (20 U/µl)
Transcriptor RT Reaction Buffer 1 ml (5x)
Protector RNase Inhibitor 100 µl (40 U/µl)
Deoxynucleotide Mix 200 µl (10 mM each)
Anchored-oligo(dT) Primer 200 µl (50 µM)
Random Hexamer Primer 200 µl (600 µM)
Water, PCR-grade 2x1ml
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