TATAA Interplate Calibrator (IPC) – Inter-Variability Control for qPCR

TATAA IPC is a purpose-designed control material developed by TATAA Biocenter to evaluate and manage inter-assay variability in quantitative PCR (qPCR) workflows. It enables researchers to benchmark and monitor variations introduced during sample preparation, pipetting, cycling, or instrument performance.

Unlike positive or negative controls, the IPC is not used to detect target amplification, but rather to provide a consistent, well-characterized reference signal that helps normalize experimental variability between different runs, batches, or setups.

Variation Compensation

The TATAA Interplate Calibrator (IPC) makes it possible for you to compensate for variations between qPCR runs. For practical reasons many qPCR studies involve the use of samples that are processed in more than a single batch or in which the sample set is extended over time. Even over a short time period, variation between qPCR processing runs is observed due to different baseline subtractions and threshold settings.

When to use TATAA Interplate Calibrator

The bias is NOT introduced when using the “all samples” or “all assays” plate layout or when performing ΔΔCq based analysis, but it is the “mixed” layout for which interplate calibration is needed (Figure 1). The TATAA IPC sample material is provided in ready-to-use aliquots and is a very stable template that is amplified with a highly robust assay. The TATAA IPC should be included in all qPCR runs. Any differences in the measured IPC Cq-values among the runs reflect the bias introduced by the instrument and can then compensated for.

TATAA Interplate Calibrator:

• Track and compare inter-assay performance across time, users, or platforms
• Control for workflow-induced variability in high-sensitivity assays
• Compatible with all standard qPCR cyclers and chemistries
• Developed and validated by qPCR experts at TATAA Biocenter

User Manual

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