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Quantify the absolute amount of human genomic DNA
When analyzing circulating cell free DNA, it is critical the sample contains enough genomic DNA for the measurements to be accurate. This is particularly important when assaying for rare mutations or variants, as the factor limiting sensitivity often is the total number of DNA copies in the sample.
TATAA calibrated human genomic DNA standard
TATAA genomic DNA standard is calibrated against National Institute of Standards and Technology (NIST) Standard Reference Material (SRM) 2372 (“Human DNA Quantitation Standard”) and whose absolute concentration has further been validated with digital PCR using the ValidPrime assay. The TATAA calibrated human DNA standard is excellent for the determination of the absolute amount of human genomic DNA in precious samples by a comparative measurement with eg., qPCR, dPCR or NGS. For quantification of cell-free DNA and other gDNA expected to be apoptotic dPCR and qPCR assays generating short amplicons are recommended. ValidPrime is preferred when precision is given priority, while Alu assay is preferred when sensitivity is priority. Using differential assays, it is also possible to estimate the fraction of apoptotic gDNA in a sample.
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