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Vendor: TATAA Biocenter AB
The TATAA Reference Gene Panel – Rat is developed to help you select the most stable reference (housekeeping) gene for normalizing gene expression data in rat samples. Instead of relying on assumptions or generic recommendations, this panel lets you test a set of commonly used reference genes and determine which is most appropriate for your experimental conditions and tissue type.
With this kit, you can systematically evaluate reference gene stability using tools like geNorm and ensure reliable, reproducible qPCR data.
Description
For all gene expression studies using qPCR, it is necessary to compensate for differences between samples due to material losses, differences in RT yields and PCR inhibition. Normalization should include a reference gene, but can also be complemented by identical sample-input amounts. The reference gene should have constant expression in all the samples compared. There is no universal control gene, expressed at a constant level under all conditions and in all tissues, hence the choice of reference gene should be validated for each new study.The best way to choose the proper reference gene(s) is by running a panel of potential genes on a number of representative test samples. The most appropriate gene(s) for normalization are chosen in each case.ContentAssays for 12 of the most common reference genes for gene expression studiesEnough for 100 reactions per gene (25 µl qPCR reaction)Positive Control DNAThe Reference Gene Panels are available for Human, Mouse and Rat. Genes with varying cellular functions and expression levels have been selected to reduce the risk of using co-regulated genes. The primers have been designed to be exon-spanning where possible, and to give low levels of primer dimers
User Manual
Reference gene selection kit
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