Reference Gene Panel – Rat | Select the Best Normalization Gene for qPCR

The TATAA Reference Gene Panel – Rat is developed to help you select the most stable reference (housekeeping) gene for normalizing gene expression data in rat samples. Instead of relying on assumptions or generic recommendations, this panel lets you test a set of commonly used reference genes and determine which is most appropriate for your experimental conditions and tissue type.

With this kit, you can systematically evaluate reference gene stability using tools like geNorm and ensure reliable, reproducible qPCR data.

Description

For all gene expression studies using qPCR, it is necessary to compensate for differences between samples due to material losses, differences in RT yields and PCR inhibition. Normalization should include a reference gene, but can also be complemented by identical sample-input amounts. The reference gene should have constant expression in all the samples compared. There is no universal control gene, expressed at a constant level under all conditions and in all tissues, hence the choice of reference gene should be validated for each new study.
The best way to choose the proper reference gene(s) is by running a panel of potential genes on a number of representative test samples. The most appropriate gene(s) for normalization are chosen in each case.

Content
Assays for 12 of the most common reference genes for gene expression studies
Enough for 100 reactions per gene (25 µl qPCR reaction)
Positive Control DNA
The Reference Gene Panels are available for Human, Mouse and Rat. Genes with varying cellular functions and expression levels have been selected to reduce the risk of using co-regulated genes. The primers have been designed to be exon-spanning where possible, and to give low levels of primer dimers

• Includes multiple commonly used rat reference genes
• Designed for SYBR® Green-based qPCR and probe based qPCR
• Compatible with geNorm for gene stability ranking
• Useful across a wide range of rat tissues and cell types
• Enhances qPCR normalization and result accuracy

User Manual 

Reference gene selection kit

Other quality controls from TATAA