TATAA GrandScript cDNA SuperMix – One-Tube cDNA Synthesis Solution

TATAA GrandScript cDNA SuperMix is a one-tube, ready-to-use solution for efficient cDNA synthesis directly from RNA samples. The fully premixed format eliminates pipetting steps and minimizes technical variation, ensuring high sensitivity, reproducibility, and convenience.

Optimized for reverse transcription across a broad RNA input range, GrandScript SuperMix is ideal for RT-qPCR, biomarker validation, gene expression profiling, and studies involving low-input or degraded RNA (e.g., from FFPE or biofluids).

The TATAA GrandScript cDNA SuperMix is a sensitive and easy to use 1-tube solution for cDNA synthesis.

The Supermix provides all the necessary components for cDNA synthesis except RNA template. The optimized mix of random and oligo(dT) primers and engineered MMLV reverese transcriptase provides a robust, consistent and unbiased first strand synthesis over a broad range of RNA template concentrations. The resulting cDNA product is directly compatible with real-time PCR methods. Features Easy-to-use, one-tube format High sensitivity and broad dynamic range Unbiased cDNA synthesis - complete 5’ to 3’ RNA sequence representation Ideal for high-throughput applications

Content

5X reaction buffer containing optimized concentrations of MgCl2, dNTPs, recombinant RNase inhibitor protein, GrandScript reverse transcriptase, random primers, oligo(dT) primer and stabilizers

• True one-tube format – all components pre-mixed for maximal convenience
• High reverse transcription efficiency
• Minimal handling reduces variability
• Compatible with a wide range of RNA inputs and sample types
• Validated for both standard and multiplex RT-qPCR assays
  

User Manual

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