ArcticZymes Heat Labile Exonuclease I (HL-ExoI) – ssDNA Removal Enzyme

HL-ExoI (Heat Labile Exonuclease I) from ArcticZymes selectively digests single-stranded DNA (ssDNA) such as primers, oligos, and unincorporated nucleotides.

The enzyme is fully inactivated by mild heat treatment, making it ideal for post-PCR and NGS library cleanup workflows where RNA and double-stranded DNA need to be preserved. Its heat-lability simplifies workflows by eliminating the need for additional purification steps.

HL-ExoI is a 3’-5’ exonuclease, specific for single stranded DNA. HL-ExoI is active at temperatures ?25°C, and inactivated by 1 min incubation at 80°C, or 15 min at 60°C

• Recombinant Heat Labile Exonuclease from ArcticZymes
• Degrades ssDNA, including primers and oligonucleotides
• Ideal for post-PCR and post-reverse transcription cleanup
• Fully heat-inactivated—no purification step needed
• Compatible with NGS, qPCR, and molecular diagnostics
• Gentle on double-stranded DNA and RNA

More products from ArcticZymes