TATAA GrandScript cDNA Synthesis Kit – High-Efficiency Reverse Transcription

The TATAA GrandScript cDNA Synthesis Kit is a high-performance solution for converting RNA to cDNA with exceptional sensitivity and reproducibility. Optimized for RT-qPCR, it efficiently transcribes even low-input or partially degraded RNA into high-quality cDNA suitable for gene expression profiling, biomarker discovery, and diagnostics.

The kit includes all necessary reagents for a flexible and reliable reverse transcription protocol and is ideal for use with total RNA, mRNA, or viral RNA extracted from a broad range of biological materials.

The TATAA GrandScript cDNA Synthesis Kit is a sensitive and easy to use solution for cDNA synthesis.

TATAA GrandScript cDNA Synthesis Kit provides all the necessary components for cDNA synthesis except enzyme and RNA template. The optimized mix of random and oligo(dT) primers provides a robust consistent and unbiased first strand synthesis over a broad range of RNA template concentrations.

TATAA GrandScript cDNA Synthesis enzyme is a mixture of engineered MMLV RT and ribonuclease inhibitor protein. The resulting cDNA product is directly compatible with real-time PCR methods. Features High sensitivity - optimized for two-step quantitative RT-PCR Broad dynamic range Unbiased cDNA synthesis - complete 5’ to 3’ RNA sequence representation Easy reaction assembly

Content

• TATAA GrandScript cDNA Synthesis Mix: 5x concentrated solution of optimized buffer, magnesium, oligo(dT), random primers and dNTPs
• TATAA GrandScript RT enzyme
• Nuclease- free water

Overview

• High-yield, high-fidelity cDNA synthesis
• Flexible protocol with customizable priming options
• Compatible with a wide input range (low to high RNA amounts)
• Designed for sensitive and reproducible RT-qPCR performance
  

User Manual

Other cDNA synthesis kits from TATAA Biocenter