Shrimp-Alkaline-Phosphatase-(SAP)

The main advantages of SAP:
100% heat-inactivated at 65°C
Removes 5’-phosphates from DNA, RNA, dNTPs and proteins.
Works in restriction enzyme buffers.
Used in PCR clean-up.

Developed at ArcticZymes, first launched in 1993. Originates from the Arctic Shrimp (Pandalus borealis), from 2010 it has been produced as a recombinant version (rSAP). rSAP is better suited than native SAP (nSAP) for newer, more sensitive technologies.

Shrimp Alkaline Phosphatase (rSAP) has become one of today’s most-selling DNA modifying enzymes due to the added convenience through a complete heat inactivation. While most other alkaline phosphatases (from E. coli or Calf intestine) must be removed by extraction procedures, rSAP is completely inactivated after 15 minutes at 65°C. rSAP works well in common buffers without the requirement for other additions.

Main advantages with SAP
Very high specific activity
100% heat-inactivated at 65°C
Removes 5’-phosphates from DNA, RNA, dNTPs and proteins
May be added directly to restriction enzyme digests
No vector purification necessary
Requires no supplemental zinc or other additives for activity
Works directly in many different buffers
Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis