Double-strand specific DNase (dsDNase)

dsDNase is inactivated at 65°C.

This is an all-purpose double-strand specific DNase by ArcticZymes, which is used in our PCR decontamination kit.

Heat inactivation

dsDNase can be heat inactivated by heat treatment at 20 min at 60?C, or 10 min at 65?C, in the presence of 1 mM DTT.

Specifications

Unit definition

One Unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCl2 (Kunitz, 1950).

Specific activity

475 000 Kunitz Units/mg.
pH optimum
pH 7.5 (in Tris-HCl).
Storage buffer
20 mM Tris-HCl pH 7.5, 2 mM MgCl2, 10 mM NaCl, 0.01% (v/v) Triton X-100, 50% (v/v) glycerol.
Reaction buffer
20 mM Tris-HCl pH 8.0, 5 mM MgCl2. Dependent on magnesium ions for activity. Dependent on 1 mM DTT for inactivation.

Storage

Minimum shelf life is 2 years at -20°C. Storage at 4°C is possible for at least 6 months. The enzyme also tolerates multiple freeze-thaw cycles.

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