LUTHOR High-Definition Single-Cell 3' mRNA-Seq Kit


Vendor: Lexogen

LUTHOR High-Definition Single-Cell 3' mRNA-Seq Kit

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LUTHOR High-Definition Single-Cell 3′ mRNA-Seq Library Prep Kit

LUTHOR HD redefines the approach to single-cell RNA sequencing by offering an RNA-amplification-based kit able to detect virtually every single mRNA molecule present in the cell.

Like QuantSeq FWD, LUTHOR HD is a 3′ end mRNA-Seq kit that uses oligo(dT) primers to target polyadenylated RNAs and generate reads mapping to the 3′ end of transcripts. The two kits, however, use different protocols and technologies and are developed for different sample input amounts. While LUTHOR HD is designed for inputs ranging from 1 ng to 10 pg (equivalent to 100 cells to 1 cell) and even lower*, QuantSeq FWD is ideal for inputs ranging from 1 ng to 500 ng of total RNA.

LUTHOR HD reaction allows to amplify minuscule amounts of RNA material and to obtain a full understanding of each cell’s gene expression status.

HD scRNA-Seq applications

High-Definition single-cell RNA sequencing works best with ultra-low initial inputs (as low as 10 pg RNA). By seeing more genes than classical bulk scRNA-Seq methods, it expands transcriptomic analysis into new scientific applications.

  • High-definition single-cell differential gene expression analysis
  • Detailed characterization of cell subpopulations after larger, less sensitive screenings (bulk scRNA-Seq)
  • Rare cell RNA analysis (e.g., circulating tumor cells (CTCs) or innate lymphoid cells (ILCs))
  • Subcellular (cytoplasm, organelle) RNA analysis
  • Single nucleus RNA-Seq

LUTHOR High-Definition Single-Cell 3’ mRNA-Seq kits are provided with all necessary reagents to perform the full library preparation protocol:

  • Cell lysis (optional)
  • THOR reaction
  • In vitro transcription (IVT)
  • Reverse transcription
  • Endpoint PCR
  • Purification reactions, with magnetic beads

Cat. No. 221 additionally provides the UDI Set B1, designed to perform error correction and maximize the reads recovered from your sequencing run.