CRISPR-Test Assay Kits

Accurately Assess Cas9 Activity in Cells

• Measure functional activity of Cas9 nuclease, dCas9-repressor, or dCas9-activator in any cell line
• Obtain quantitative FACS-based read-outs
• Get complete, easy-to-use kits with ready-to-transduce lentiviral particles

The CRISPR-Test assay kits contain a mix of lentiviral particles with both green fluorescent and red fluorescent protein (GFP and RFP). The GFP fluorescent marker responds to the specific Cas9 activity the Kit measures whether nuclease (CRISPRu or CRISPR KO), gene repression (CRISPRi), or gene activation (CRISPRa). The RFP fluorescence provides a normalization control.

How Does it Work?

1.Transduce your test population of putative Cas9, dCas9-activator, or dCas9-repressor cells.

2. Determine by flow cytometry the ratio of green tored fluorescent cells.
3. After 4-6 days, measure the ratio of green to red fluorescent cells again.
4. Cas9 activity correlates with the difference in the initial vs. end-point ratios of green vs. red cells.