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Accurately Assess Cas9 Activity in Cells
The CRISPR-Test assay kits contain a mix of lentiviral particles with both green fluorescent and red fluorescent protein (GFP and RFP). The GFP fluorescent marker responds to the specific Cas9 activity the Kit measures whether nuclease (CRISPRu or CRISPR KO), gene repression (CRISPRi), or gene activation (CRISPRa). The RFP fluorescence provides a normalization control.
How Does it Work?
1.Transduce your test population of putative Cas9, dCas9-activator, or dCas9-repressor cells.
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