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Vendor: Norgen Biotek
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Purification and enrichment of intact plasma/serum exosomes for functional studies
Bind and elute all RNA irrespective of size or GC content, without bias
Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA?
Versatile plasma/serum input volume (50 µL - 1 mL)
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents, nor overnight incubation required
Compatible with plasma/serum from any species
Pure exosomes are purified and are free-from any other RNA-binding proteins
Plasma/Serum Exosome Purification and RNA Isolation Mini Kit
Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of exosomal RNA and free-circulating RNA from different plasma/serum sample volumes ranging from 50 µL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Minimum Plasma/Serum Input: 50 ?L
Maximum Plasma/Serum Input: 1 mL
Size of Exosomes Purified: 40 nm - 150 nm
Size of RNA Purified: All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume: 50-100 ?L
Time to Complete 10 Purifications: 35 - 40 minutes
Average Yields*: Variable depending on specimen
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