Design and Validation of q/dPCR assays

A109###

Vendor: TATAA Biocenter AB

Design and Validation of q/dPCR assays

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Overview

When designing assays to measure sequence variants, the ValidPrime target sequence serves as internal standard making it possible to accurately mix the two standards at any ratio.

Contents

  • Primers and probe for target 1 (usually WT)
  • Primers and probe for target 2 (usually variant)
  • Primers and Probes for ValidPrime
  • Positive control material for target 1 (synthetic DNA containing ValidPrime and target 1)
  • Positive control material for target 2 (synthetic DNA containing ValidPrime and target 2)

Assays will be validated for one dPCR instrument of choice (see below), using recommended master mix by manufacturer. 

  • Roche Digital LightCycler
  • ThermoFisher Absolute Q
  • Qiagen Qiaquity
  • Bio-Rad QX200
  • Stilla Naica