CelluLyser™ Lysis and cDNA Synthesis Kit – From-Cell to RT-qPCR Workflow

The CelluLyser™ Lysis and cDNA Synthesis Kit provides a complete solution for generating cDNA directly from lysed cells, eliminating the need for RNA purification. Designed for fast, accurate RT-qPCR analysis, this kit is ideal for workflows where sample input is limited, or throughput and reproducibility are critical.

The integrated workflow combines gentle cell lysis with a highly efficient reverse transcription reaction, optimized to deliver reproducible cDNA yields suitable for downstream gene expression profiling or validation studies.

Rapid and sensitive lysis and reverse transcription of small cell samples in a single tube

The optimized CelluLyser™ buffer and powerful reverse transcription reagents maximize the yield of cDNA, making CelluLyser™ ideal for studies of small samples intended for real-time PCR. With the possibility to generate cDNA directly from cell lysate without isolating RNA, considerable amount of time is saved compared to standard RNA purification. 

Description

The CelluLyser™ Lysis and cDNA Synthesis kit enables you to analyze small samples from 10 000 cells down to a single cell with minimal losses and hands-on time. The CelluLyser™ kit can be used with collected cells (FACS sorted, patch clamp collected cells etc.), as well as cultured cells (both adherent and in solution), and has successfully been tested on a number of different mammalian cell lines. 

To avoid losses of material the entire procedure can be performed in a single tube without any sample transfer. Also, 100% of the total RNA content may be used from lysis to cDNA synthesis. The CelluLyser™ procedure is rapid and straightforward. Cells are lysed in only 10 minutes in the optimized CelluLyser™ buffer, and thereafter sensitive cDNA synthesis is performed directly on the cell lysate. 

Contents

The reagents provided are sufficient either for 250 lysis reactions and 100 cDNA synthesis reactions of 1 - 2 000 cells, or for 100 lysis reactions and 40 cDNA synthesis reactions of 2 000 - 10 000 cells. 

• CelluLyser™ buffer: 1.25 ml (1X) 
• cDNA synthesis reagents (Roche Transcriptor First Strand cDNA Synthesis kit): 
• Transcriptor Reverse Transcriptase 50 µl (20 U/µl) 
  
• Transcriptor RT Reaction Buffer 1 ml (5x) 
• Protector RNase Inhibitor 100 µl (40 U/µl) 
• Deoxynucleotide Mix 200 µl (10 mM each) 
• Anchored-oligo(dT) Primer 200 µl (50 µM) 
• Random Hexamer Primer 200 µl (600 µM) 
• Water, PCR-grade 2x1ml 

Key Features

•     All-in-one solution: Cell lysis and reverse transcription in a single workflow
•     No RNA purification required
•     Compatible with low-input and precious samples
•     High-quality cDNA for accurate and sensitive RT-qPCR
•     Simplified protocol for high-throughput applications

More quality reagents from TATAA Biocenter