ValidPrime®

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Validprime kit:
459.00 EUR
209.00 EUR
359.00 EUR
159.00 EUR
459.00 EUR
209.00 EUR
359.00 EUR
159.00 EUR
479.00 EUR
229.00 EUR
399.00 EUR
199.00 EUR
459.00 EUR
209.00 EUR
379.00 EUR
179.00 EUR

Information

Control for genomic background

Description

ValidPrime® replaces the need to perform no reverse transcriptase (RT(-)) controls to test for the presence of genomic DNA (gDNA) for all samples in your real-time quantitative PCR (qPCR) profiling.

In an expression profiling experiment based on m samples and n assays, traditional set up requires m RT(-) reactions plus m x n qPCR controls.

When using ValidPrime® only m + n + 1 controls are needed.

ValidPrime® will minimize the amount of control reactions and hence your costs as well as your efforts.

Features

  • Offers a cost-efficient alternative to RT(-) controls
  • Accurately validates qPCR assays in terms of their sensitivity to gDNA
  • Allows correction for gDNA-derived signals
  • Reduces the need for DNase treatment

 

Use ValidPrime®

  • as a control for gDNA background in qPCR
  • for normalization of samples to cell copy number
  • as an endogenous control for CNV applications

 

ValidPrime® is an assay to test for the presence of gDNA in test samples and when combined with a gDNA control sample, replaces all RT(-) controls. ValidPrime® is highly optimised and specific to a non-transcribed locus of gDNA that is present in exactly one copy per haploid normal genome. The sequence detected by the ValidPrime® assay has no transcriptional activity and is not present in pure cDNA preparations. Therefore, ValidPrime® measures the number of genomic copies present in a sample.

In an expression profiling experiment the ValidPrime® assay is added to the list of assays and the gDNA control is added to the list of samples. From the combined measurements of the ValidPrime® assay and the gene of interest (GOI) assays on all samples and on the gDNA control, the genomic background contribution to all RT-qPCR measurements can be assessed. ValidPrime® replaces the need to perform RT(-) controls for all reactions and makes RT-qPCR profiling easier and substantially cheaper.

The assay has very high PCR efficiency (E > 90% in tested commercial master mixes) and produces negligible amount of primer dimer products. Limit of detection (LOD) is estimated to 4 copies of DNA (0.01 ng of DNA) and limit of quantification (LOQ) is estimated to 32 copies
of DNA (0.08 ng of DNA).

For ValidPrime® Human, ValidPrime® Mouse, and ValidPrime® Rat, a gDNA standard is provided which can be used to test the sensitivity of qPCR assays for gDNA background. ValidPrime® Vertebrate does not include a gDNA standard, since the standard needs to be specific for the species analysed and arranged by the user.

(TATAA)

Specifications

Partno: A105P10
Partno: A105P25
Partno: A105S10
Partno: A105S25
Partno: A106P10
Partno: A106P25
Partno: A106S10
Partno: A106S25
Partno: A107P10
Partno: A107P25
Partno: A107S10
Partno: A107S25
Partno: A108P10
Partno: A108P25
Partno: A108S10
Partno: A108S25

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