•  
  • A qPCR amplification plot with no inhibition as the Spike control in water and in sample matrix shows the same Cq value and efficiency
  • The qPCR inhibition is seen on the lower slope of the amplification curve. If the qPCR is inhibited, results are unreliable.

TATAA Universal DNA Spike I

1 2 3 4 5
(5/5) Based on 2 rating.
Reactions/SYBR or Probe:
699.00 EUR
699.00 EUR
655.00 EUR
205.00 EUR
205.00 EUR
195.00 EUR

Information

Control for Inhibition of the qPCR - the risk of compromised RT-qPCR results


The Universal DNA Spike from TATAA Biocenter is an easy to use and very effective tool for quality control of the qPCR experimental workflow. DNA Spike is used in the qPCR reaction to detect any inhibition. In addition TATAA Universal RNA Spike I and II can be used to detect any inhibition during the cDNA synthesis reaction.

TATAA Universal DNA Spike for quality Control


The Universal DNA Spike has a synthetic sequence that is not present in any known living organism. This DNA sequence is 1000 base pairs in length. The Spike assay, which is very robust and is optimized for high sensitivity for inhibition, amplifies a 300-base region towards the 3’ end of the synthetic transcript.
The measured Cq and the shape of the amplification curve reflect inhibition. If the Cq value is greater in an experimental sample than in the control then the analytical process of that experimental sample is inhibited (Figure 2). The magnitude of the difference between these Cq values reflects the degree of inhibition.

Specifications

Partno: DS10PFI
Partno: DS10PVI
Partno: DS10SI
Partno: DS25PFI
Partno: DS25PVI
Partno: DS25SI

Opinions

Add review

zwWF
Code from Captcha-image:

Rating (0)

You may also be interested in

  • TATAA Biocenter
    TATAA PreAmp GrandMaster® Mix is a 2x concentrated ready to use pre-amplifica...
    from 365.00 EUR
  • TATAA Biocenter
    TATAA GrandScript cDNA Synthesis Kit provides all the necessary components fo...
    from 110.00 EUR
  • TATAA Biocenter
    The TATAA Interplate Calibrator makes it possible for you to compensate for v...
    from 195.00 EUR
  • TATAA Biocenter
    ValidPrime® replaces the need to perform no reverse transcriptase (RT(-)) con...
    from 159.00 EUR
  • Other products of the same brand

  • TATAA Biocenter
    Detect and quantify the amount of human Y-chromosome
    Art. no. Y109P10
    150.00 EUR
  • TATAA Biocenter
    The easy-to-use qPCR fastmix for all your gene expression and genotyping expe...
    from 310.00 EUR
  • TATAA Biocenter
    The easy-to-use qPCR fastmix for all your gene expression experiments
    from 310.00 EUR
  • TATAA Biocenter
    Assays designed for a microRNA target specified by the customer. To place an...
    Art. no. Custom design of new assays
    No price