Rapid quantification of rare target sequences in dilute samples

Description

TATAA One-Step Fast GrandMaster® Kit is a 4X concentrated fastmix which allows analysis of larger volumes of sample. This results in an ability to quantify rare target sequences in dilute samples.

TATAA One-Step Fast GrandMaster® Kit has been developed for fast and highly sensitive qRT-PCR of a wide range of RNA targets. It is optimized for use with conventional 5’ hydrolysis probes and allows for unbiased amplification of low copy transcripts in the presence of high copy reference genes.

Content

TATAA One-Step Reverse Transcriptase - Optimized 20X formulation of recombinant MMLV reverse transcriptase for One-Step Fast qRT-PCR
TATAA GrandMaster® One-Step Fast Master Mix*
4x reaction buffer containing optimized concentrations of MgCl2, dNTPs, Taq DNA polymerase and stabilizers
Nuclease-Free Water

The The TATAA SYBR® GrandMaster® Mix can be ordered without ROX, with low ROX or with ROX depending on the instrument used for the analysis.can be ordered without ROX, with low ROX or with ROX depending on the instrument used for the analysis.

The number of reactions is based on 20μl qPCR-reactions.