TATAA DeltaAmp Quality assay

1 2 3 4 5
(5/5) Based on 10 rating.
deltaAmp kits:
235.00 EUR
180.00 EUR
235.00 EUR
180.00 EUR
235.00 EUR
180.00 EUR

Information


A new molecular method to assess RNA/DNA integrity with qPCR


By using paired qPCR assays that produce amplicons of different lengths from the same target the quality of the RNA can be evaluated. In intact mRNA the Cq's of the short and long amplicons are virtually the same, while for fragmented RNA the Cq of the longer amplicon is increased relative to that of the shorter. The TATAA DeltaAmp Quality Assays consist of short, medium and long qPCR assays that have a common reverse primer.  

Three different targets; Beta-2-Microglobulin (B2M), 18S rRNA (18S) and an Endogenous RNase Resistant marker (ERR) are available for SYBR or Probe based assays.

 

Background


Integrity of the mRNA has major impact on the quality of measured expression levels. This is independent of the measurement technique, may it be Next Generation Sequencing (NGS), Quantitative Real-Time PCR (qPCR) or microarray profiling. If mRNA is highly degraded or damaged, measured data will be very unreliable and the whole study is likely a waste of time and money. It is therefore common strategy to test the quality of RNA in samples before conducting large and expensive studies.

Instructions for use


The TATAA DeltaAmp Quality Assays are intended to be used to assess the quality of RNA in human samples by qPCR measurement and consist of short, medium and long qPCR assays. The difference in Cq's of a longer and shorter assay for the same target reflect the integrity of the RNA in the sample and can be evaluated by the score ΔAmp = CqAmplicon1 – CqAmplicon2 as an indicator of RNA integrity.

As a second approach amplicon 1 could be the amplicon of an mRNA marker, such as a reference gene (for example the B2M assay), that has normal sensitivity to RNases and amplicon 2 the amplicon of the short assay for the ERR marker. Also in this case the ΔAmp = CqAmplicon1 – CqAmplicon2 can be used as an indicator of RNA integrity.

For optimal use of these assays it is recommended to compare the ΔAmp-score of the unknown samples to that of sample(s) of known good quality.

Specifications

Partno: DAB200F
Partno: DAB200S
Partno: DAE200F
Partno: DAE200S
Partno: DAS200F
Partno: DAS200S

Opinions

Add review

6GrFR
Code from Captcha-image:

Rating (0)

You may also be interested in

  • TATAA Biocenter
    The TATAA Probe GrandMaster® Mix is a 2x concentrated ready to use fastmix wh...
    from 480.00 EUR
  • TATAA Biocenter
    Control for Inhibition in the RT-PCR reaction - The Risk of Compromised RT-qP...
    from 195.00 EUR
  • TATAA Biocenter
    Control for Primer Dimers in Probe based qPCR assays.
    Art. no. B102-6
    209.00 EUR
  • TATAA Biocenter
    Find the Most Stable Reference Genes with TATAA Biocenter's Reference Gene Pa...
    from 395.00 EUR
  • Other products of the same brand

  • TATAA Biocenter
    Detect and quantify the mount of human Y-chromosome The human Y-chromosome i...
    Art. no. Y109P10
    150.00 EUR
  • TATAA Biocenter
    The easy-to-use qPCR fastmix for all your gene expression and genotyping expe...
    from 251.00 EUR
    ( 310.00 EUR)
  • TATAA Biocenter
    The easy-to-use qPCR fastmix for all your gene expression experiments
    from 251.00 EUR
    ( 310.00 EUR)
  • TATAA Biocenter
    Assays designed for a microRNA target specified by the customer. To place an...
    Art. no. Custom design of new assays
    No price