SENSE Total RNA-Seq Library Prep Kit for Illumina
- Strand-specificity >99.9 %
- Ready-to-sequence libraries in 3.5 hours
- 0.5 ng of rRNA-depleted or poly(A) selected RNA input incl. from low-quality RNA and FFPE samples
The strand-displacement stop/ligation technology used in SENSE generates fewer antisense artifacts by omitting RT artifacts, and therefore avoids spurious second strand cDNA synthesis, which may result in the detection of false antisense transcription. Thereby an exceptional (99.9%) strand-specificity and reduced experimental noise is reached, enabling the detection and quantification of antisense transcripts with high confidence.
With the 96 external barcodes included in the kit libraries can be easily multiplexed.
Streamlined Ribosomal RNA Depletion
For efficient utilization of RNA-Seq reads highly abundant ribosomal RNA should be eliminated prior library preparation. SENSE Total RNA-Seq Kit is available in combination with RiboCop rRNA Depletion Kit. This is a complete solution for RNA-Seq library preparation, starting with total RNA and finishing with a ready-to-sequence library.
NGS-ready libraries can be produced from poly(A) selected or rRNA-depleted RNA samples in under 3.5 hours with less than 50% hands-on time, allowing RNA extraction, library preparation and quality control to be performed in one day.
Flexible Input Requirements
The typical input amount of poly(A) or rRNA-depleted RNA is 0.5 ng – 500 ng. SENSE Total library generation can furthermore be done on intact as well as highly degraded RNA samples (including FFPE).
Libraries for Different Sequencing Read Length
For good representation and even coverage of all transcripts in your experiment the library should have a size suitable for the chosen sequencing read length. The size of SENSE libraries can be adjusted by simply modulating the purification steps.
The SENSE Total RNA-Seq kit is based on Lexogen’s proprietary strand-displacement stop/ligation technology and needs poly(A) selected or rRNA-depleted RNA input.