SENSE mRNA-Seq Library Prep Kit for Ion Torrent
- Sense kit for Ion Torrent:
Strand-specificity >99.9 %
- Fragmentation-free library generation
- 1 ng of total RNA input
- Ready-to-sequence libraries in under 5 hours
The strand-displacement stop/ligation technology used in SENSE generates fewer antisense artifacts which can be produced by template-switching in protocols which utilize RNA or cDNA fragmentation. This results in exceptional (>99.9%) strand-specificity and reduced experimental noise, enabling the detection and quantification of antisense transcripts with high confidence.
NGS-ready libraries can be produced from total RNA samples in under 5 hours with less than 50% hands-on time, allowing RNA extraction, library preparation and quality control to be performed in one day.
No additional kits or reagents for poly(A) RNA selection, library amplification, size selection or purification are required or barcodes.
Low Amount of Input Total RNA
The typical amount of input total RNA is 1 ng – 2 µg.
Efficient rRNA Elimination
SENSE mRNA-Seq Library Prep includes the Poly(A) RNA Selection Kit (Cat. No. 039) which virtually eliminates cytoplasmic ribosomal RNA (<0.001% of total reads from Universal Human Reference RNA) and removes the need for additional selection or depletion kits, saving you time and money.
Different Sequencing Read Length
For good representation and even coverage of all transcripts in your experiment the library should have a size suitable for the chosen sequencing read length. The size of SENSE libraries for Illumina can be adjusted by simply modulating appropriate buffers during RT/ligation and purification steps.
SENSE mRNA-Seq Library Prep Kits are available for Illumina, Ion Torrent, and SOLiD sequencing platforms.
SENSE libraries can be multiplexed with very well balanced barcode sequences. Learn more about barcodes for multiplexing on different sequencing platforms.
The SENSE kit features an all-in-one mRNA-Seq library preparation protocol. Most of the procedure is performed on magnetic beads, making it amenable to automation and decreasing purification time. The SENSE protocol has a simple workflow consisting of just 3 major steps.