RiboCop rRNA Depletion Kit for Mixed Bacterial Samples (META)
RiboCop for Bacteria
RNA extracted from bacterial species comprises up to 98 % of the ribosomal RNAs (rRNA), presenting a unique challenge especially when analyzing the transcriptome capacity from complex bacterial communities. Lexogen’s RiboCop rRNA Depletion Kits for Bacteria efficiently remove 23S, 16S, and 5S rRNA from mixed bacterial samples and monocultures.
RiboCop is offered as a stand-alone kit with the option to choose from three optimized Probe Mixes for depletion of rRNA from mixed bacterial samples (META, Cat. No. 125) or from Gram negative or Gram positive bacteria (G- or G+, Cat. No. 126 and 127, respectively) grown in monoculture.
RiboCop is compatible with all random primed library prep kits such as the CORALL Total RNA-Seq Library Prep Kit (Cat. No. 095) or adapter ligation protocols such as the Small RNA-Seq Library Prep Kit (Cat. No. 052).
Efficient Elimination of rRNA
RiboCop allows efficient removal of rRNAs. RiboCop for Human/Mouse/Rat (HMR, Cat. No. 037) removes cytoplasmic 28S, 18S, 5.8S, 45S, 5S, as well as mitochondrial mt16S, mt12S ribosomal RNA sequences in human, mouse, and rat samples. RiboCop for Bacteria removes 23S, 16S, and 5S rRNA from Gram positive and Gram negative bacterial species as well as from mixed bacterial samples. Sequencing depth is thus preserved for relevant RNA species.
The RiboCop protocol takes 1.5 hours to complete (30 minutes hands-on time). No enzymatic reactions or mechanical shearing steps are involved, leaving full-length transcripts intact for downstream processing.
Flexible Input Requirements
RiboCop is suitable for rRNA depletion of 1 – 1,000 ng total RNA input amounts and is compatible with intact or degraded RNA (including FFPE) samples. Specific input recommendations apply for certain sample types and are described in the respective User Guides.
Magnetic beads are used for depletion and purification steps rendering the protocol completely automation-friendly.
Various Downstream Applications
RiboCop rRNA Depletion Kits are offered as stand-alone kits in different kit sizes for use with any downstream application. For convenience, RiboCop for Human/Mouse/Rat (HMR) is also available in combination with the CORALL Total RNA-Seq Library Prep Kit (Cat. No. 096).