HOME » NGS » DNA » TATAA NGS Library Quantification Kit

TATAA NGS Library Quantification Kit

1 2 3 4 5
(5/5) Based on 2 rating.
359.20 EUR Ordinary price (449.00 EUR)

Information

Background

This kit is based on absolute quantification of your NGS libraries using Illumina adaptor primers and qPCR. The amplifiable fraction of the library will get amplified, and the quantity will be estimated using a standard curve. The goal of the analysis is to find the optimum concentration for the NGS library, since over- or underestimation of the concentration may compromise the sequencing capacity.

- Underestimation of the concentration → Overloading of the flow cell → Poor cluster resolution
- Overestimation of the concentration → Insufficient template amount → Low cluster density

Six DNA standards of known concentrations are part of the kit for generation of a robust standard curve. They will generate PCR products of 415 bp. After diluting your NGS library of unknown concentration 10 000 times and performing qPCR analysis, the library concentration is assessed. PCR based library preps yield mostly concentrations of 1-50 nM.

Contents

• NGS library assay primers (V = 250 μl primer mix, c = 10 μM per primer, Blue cap)
• TATAA SYBR GrandMaster Mix LowROX (V = 2 · 1250 µl, c = 2x)
• NGS library standards: 10-fold diluted Standard 1-6, dsDNA in TE buffer (V = 40 µl per standard)

 

Specifications

Partno: TA20-NGSQ

Opinions

Add review

v84M
Code from Captcha-image:

Rating (0)

Product documents

Other products of the same brand

  • TATAA Biocenter
    Alu elements are short stretches of about 300 bp that are unique to the human...
    80.75 EUR
    ( 95.00 EUR)
  • TATAA Biocenter
    Robust and sensitive cDNA synthesis with user-defined RT priming
    from 126.65 EUR
    ( 149.00 EUR)
  • TATAA Biocenter
    Quantitative measurement of the biomining bacteria Acidithiobacillus caldus, ...
    Art. no. BQ-B01
    No price
  • TATAA Biocenter
    Detect and quantify the amount of human Y-chromosome
    Art. no. Y109P10
    150.00 EUR