The BioDynami NGS DNA Library Prep Kit (for Ion Torrent platform) was developed for construction of high quality libraries for next generation sequencing using Ion Torrent platform. The kit uses double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (dsDNA fragmentase etc.) and physical methods (sonication, nebulization etc.). Our unique technology increases library conversion rate and eliminates insert concatemer ligation.
- all steps in one tube
- two reaction steps followed by one purification step
- hands-on time: ~7 min
- total time: ~1 hr
• High quality: better library conversion efficiency
• No insert concatemer ligation
• Input DNA amount: from 100 ng to 1 ug