The BioDynami NGS DNA Library Prep Kit was developed for construction of high quality libraries for next generation sequencing using illumina platform. The kit adds 3'-dT-tailed library adapters to both ends of DNA fragments efficiently. The kit uses double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (dsDNA fragmentase etc.) and physical methods (sonication, nebulization etc.).
- all steps in one tube
- two reaction steps followed by one purification step
- hands-on time: ~7 min
- total time: ~1 hr
• High quality: better library conversion efficiency
• Better coverage at difficult regions such as high GC plus repeat region
• Input DNA amount: from 100 ng to 1 ug