This kit is intended for quantitative measurement of the bacteria Acidithiobacillus caldus, Leptospirillum ferriphilum and Sulfobacillus thermosulfidooxidans. These three bacteria are used in biomining, which is the process of metal extraction from sulphide ores/concentrates with the aid of acidophilic microorganisms. The assays in the kit are designed to amplify the intended target of all strains of the selected bacteria, but no other known DNA sequence of other species. The measurement range is between 102 – 107 copies per microliter extracted fluid.
The field samples may be measured over time in independent runs. The reference sample of known concentration that is provided in the kit, in combination with a calibration curve, makes it possible to obtain quantitative measurements of all samples.
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