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PrepGEM™ Tissue

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prepGEM Tissue is a preparative method for DNA extraction - it is not a purification protocol

Its purpose is to lyse cells and to strip the DNA of nucleoproteins. The formulation creates DNA that can be used for SNiPs, STRs, quantitative, multiplex and routine PCR applications.

prepGEM tissue works well for mouse genotyping and cellcultures.

  • The prepGEM Tissue procedure can be automated using most liquid handling robots.
  • prepGEM Tissue is a preparative method for DNA extraction - it is not a purification protocol. Its purpose is to lyse cells and to strip the DNA of nucleoproteins. The formulation creates DNA that can be used for SNiPs, STRs, quantitative, multiplex and routine PCR applications.
  • For accurate yield assessment, a qPCR is recommended. The DNA produced by prepGEM is approximately 90% single-stranded. If standard fluorescent chelating dyes are to be used for quantification, then this factor should be taken into consideration.
  • As with any preparative method for nucleic acid extraction, for best results prepare and manage samples at 4ºC, or on ice, before and after extraction.
  • When storing the sample after extraction, aspirate the supernatant from the precipitated residue and store separately.
  • prepGEM is stable for 30 days at 4º C, for longer-term storage prepGEM should be stored at -20ºC.
  • For long-term storage of the extracted DNA, add TE buffer to 1x and store at -20°C.

 

Specifications

Partno: PTI001K
Partno: PTI005K
Partno: PTI0200
Partno: PTI0500

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